Research Article
MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T Cells
Figure 6
MMP9 and CCL1-knockdowned DC-CIK cells enhanced T-cell activation and prevented T-cell depletion. T cells were cocultured with DC-CIK cells transfected with MMP9 or/and CCL1 shRNAs. (a, b) The levels of T-cell-related cytokines (IL-2, IFN-γ, GZMB, and LAMP-1) were monitored through ELISA and qRT-PCR assays. (c) qRT-PCR analysis was conducted to confirm the levels of T-cell depletion indexes (PD-1, TIM3, LAG3, and CTLA4). TNormal group means T cells isolated from normal human mononuclear cells.
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