MMP9-Associated Tumor Stem Cells, CCL1-Silenced Dendritic Cells, and Cytokine-Induced Killer Cells Have a Remarkable Therapeutic Efficacy for Acute Myeloid Leukemia by Activating T Cells

Table 2

The sequence of all primers in qRT-PCR assay.

ID	Sequence (5-3)	Product length (bp)
Human
GAPDH (forward)	TGTTCGTCATGGGTGTGAAC	154
GAPDH (reversed)	ATGGCATGGACTGTGGTCAT
CHI3L1 (forward)	GAAGACTCTCTTGTCTGTCGGA	108
CHI3L1 (reversed)	AATGGCGGTACTGACTTGATG
PD-1 (forward)	CAACGGGCGTGACTTCC	297
PD-1 (reversed)	ATTGTCCCTCGTGCGGC
MMP9 (forward)	AGACCTGGGCAGATTCCAAAC	94
MMP9 (reversed)	CGGCAAGTCTTCCGAGTAGT
TNFSF15 (forward)	GCACCTCTTAGAGCAGACGG	212
TNFSF15 (reversed)	CGGAATGTGACCTGGGAGTAAAT
CCL1 (forward)	CAGCTCCATCTGCTCCAATG	92
CCL1 (reversed)	GCCTCTGAACCCATCCAACT
IDO1 (forward)	TCTCATTTCGTGATGGAGACTGC	130
IDO1 (reversed)	GTGTCCCGTTCTTGCATTTGC
ZNF264 (forward)	AGAGTCCGTAGCCATAACTCAT	202
ZNF264 (reversed)	GCAGGAGATGTGTGCTCTTAAT
SH2D1A (forward)	AGGCGTGTACTGCCTATGTG	183
SH2D1A (reversed)	TGCAGAGGTATTACAATGCCTTG
TIM3 (forward)	AGACAGTGGGATCTACTGCTG	157
TIM3 (reversed)	CCTGGTGGTAAGCATCCTTGG
LAG3 (forward)	GCCTCCGACTGGGTCATTTT	131
LAG3 (reversed)	CTTTCCGCTAAGTGGTGATGG
CTLA4 (forward)	CATGATGGGGAATGAGTTGACC	92
CTLA4 (reversed)	TCAGTCCTTGGATAGTGAGGTTC
IL-2 (forward)	TCCTGTCTTGCATTGCACTAAG	161
IL-2 (reversed)	CATCCTGGTGAGTTTGGGATTC
IFN-γ (forward)	TCGGTAACTGACTTGAATGTCCA	93
IFN-γ (reversed)	TCGCTTCCCTGTTTTAGCTGC
GZMB (forward)	TACCATTGAGTTGTGCGTGGG	124
GZMB (reversed)	GCCATTGTTTCGTCCATAGGAGA
LAMP-1 (forward)	TCTCAGTGAACTACGACACCA	151
LAMP-1 (reversed)	AGTGTATGTCCTCTTCCAAAAGC
Mouse
β-Actin (forward)	CATTGCTGACAGGATGCAGA	139
β-Actin (reversed)	CTGCTGGAAGGTGGACAGTGA
PD-1 (forward)	GGGTATCCCTGTATTGCTGC	147
PD-1 (reversed)	TCCTCATAGGCCACACTAGG
LAMP-1 (forward)	CAGCACTCTTTGAGGTGAAAAAC	103
LAMP-1 (reversed)	ACGATCTGAGAACCATTCGCA
GZMB (forward)	TCATGCTGCTAAAGCTGAAGAG	244
GZMB (reversed)	CCCGCACATATCTGATTGGTTT
IFN-γ (forward)	ATGAACGCTACACACTGCATC	182
IFN-γ (reversed)	CCATCCTTTTGCCAGTTCCTC
IL-2 (forward)	TGCGGCATGTTCTGGATTTG	205
IL-2 (reversed)	GGGCTTGTTGAGATGATGCTT
CTLA4 (forward)	CCATACAGGTGACCCAACCT	283
CTLA4 (reversed)	ACCTTGCAGAGGTACAGTCC
LAG3 (forward)	CTCAATGCCACTGTCACGTT	94
LAG3 (reversed)	GGGTTACCTCACACAACAGC
TIM3 (forward)	AGGGCGATCTCAACAAAGGA	163
TIM3 (reversed)	GTCTGAGCTGGAGTGACCTT