[Retracted] miR-4780 Derived from N2-Like Neutrophil Exosome Aggravates Epithelial-Mesenchymal Transition and Angiogenesis in Colorectal Cancer

Figure 4

miR-4780 was enriched in exosome derived from neutrophils and promotes the tumor progression of COLO205. (a) The hierarchic clustering of differentially expressed miRNAs was plotted to show a distinguishable miRNA expression profiling between N0-exo and N2-exo. Red represents relatively high expression; green represents relatively low expression. (b and c) miR-4780 and miR-513b-3p were both highly expressed in N2-exo. (d) Exosomes were labeled by PKH-67 (green), and the internalization of exosomes by COLO205 was observed. (e) Expression of miR-4780 in exosomes was measured via qPCR. (f and g) Cell apoptosis was detected by flow cytometer. (h and i) Wound healing assay for cell migration (). (j) CCK8 assay for cellular viability. (k and l) Transwell assay for cell invasion (). Error bars represent SD. ; ; .

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