A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories

Figure 2

Differences in cell composition and gene expression in GBM samples. (a) UMAP plots of tumor cells by Monocle3 method. (b) Pseudotime trajectory of tumor cells. (c) UMAP plot of tumor cells color-coded for each sample. (d) Tumor cell subset distributions across samples. (e) Differences in cell proportion of GBM samples in each tumor cluster. (f) Dot-plot heatmap of the most significant genes of each cluster in tumor cells. (g–l) Violin plots showing different expressions of GSCL, Undiff-G, Ac-G, Opc-G, Class-G, and Neo-G markers in each tumor cluster. (m) Violin plots showing previously reported markers expressed in GBM are distributed in GSCL and malignant cells. (n–s) Differences in gene expressions between GSCL and malignant cells clusters. means .

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