hucMSC-Ex Alleviates IBD-Associated Intestinal Fibrosis by Inhibiting ERK Phosphorylation in Intestinal Fibroblasts

Figure 5

hucMSC-Ex inhibits the expression of α-SMA, collagen I, collagen III, and fibronectin in colonic fibroblasts. (a) Western blot analysis of α-SMA, collagen I, collagen III, and fibronectin expression in human intestinal fibroblasts CCD18-Co after treatment with TGF-β for 48 hours. (b) Grayscale scanning of (a), and . (c) QPCR analysis of α-SMA, COL1A1, COL3A1, and FN1 expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 48 hours, , , and . (d) QRT-PCR analysis of COL1A1 expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 24 hours, . (e) QRT-PCR analysis of COL1A1 expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 72 hours, and ; (f) IF analysis of α-SMA expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 48 hours (400x). (g) IF analysis of fibronectin expression in human intestinal fibroblasts CCD-18Co after treatment with TGF-β for 48 hours (400x).

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