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Stem Cells International
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2023
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Article
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Tab 2
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Research Article
hucMSC-Ex Alleviates IBD-Associated Intestinal Fibrosis by Inhibiting ERK Phosphorylation in Intestinal Fibroblasts
Table 2
cDNA synthesis system.
Reagent
Volume
5x gDNA wiper mix
2
μ
l
10x RT mix
2
μ
l
HiScript III enzyme mix
2
μ
l
Oligo (dT)
20
VN
1
μ
l
Random hexamers
1
μ
l
RNase-free H
2
O
12
μ
l