Research Article

hucMSC-Ex Alleviates IBD-Associated Intestinal Fibrosis by Inhibiting ERK Phosphorylation in Intestinal Fibroblasts

Table 2

cDNA synthesis system.

ReagentVolume

5x gDNA wiper mix2 μl
10x RT mix2 μl
HiScript III enzyme mix2 μl
Oligo (dT)20VN1 μl
Random hexamers1 μl
RNase-free H2O12 μl