hucMSC-Ex Alleviates IBD-Associated Intestinal Fibrosis by Inhibiting ERK Phosphorylation in Intestinal Fibroblasts

Table 4

Primer sequences.

Species	Genes	Sequences	Annealing temperature
Mice	β-Actin	F: TCCTCCTGAGCGCAAGTACTCT	58°C
		R: GCTCAGTAACAGTCCGCCTAGAA
	TGF-β	F: CCAGATCCTGTCCAAACTAAGG	58°C
		R: CTCTTTAGCATAGTAGTCCGCT
	α-SMA	F: GCGGTGGCTATTCCTTCGTGACTAC	56°C
		R: CGTCAGGCAGTTCGTAGCTCTTC
	COL1A1	F: TGAACGTGGTGTACAAGGTC	58°C
		R: CCATCTTTACCAGGAGAACCAT
	COL3A1	F: AGTCGGAGGAATGGGTGGCTATC	58°C
		R: CAGGAGATCCAGGATGTCCAGAGG
	FN1	F: CTATAGGATTGGAGACACGTGG	58°C
		R: CTGAAGCACTTTGTAGAGCATG
Human	β-Actin	F: TCCTCCTGAGCGCAAGTACTCT	58°C
		R: GCTCAGTAACAGTCCGCCTAGAA
	α-SMA	F: TCGTGCTGGACTCTGGAGATGG	58°C
		R: CCACGCTCAGTCAGGATCTTCATG
	COL1A1	F: AAAGATGGACTCAACGGTCTC	60°C
		R: CATCGTGAGCCTTCTCTTGAG
	COL3A1	F: GGCTACTTCTCGCTCTGCTTCATC	58°C
		R: TCTCTATCCGCATAGGACTGACCAAG
	FN1	F: AATAGATGCAACGATCAGGACA	58°C
		R: GCAGGTTTCCTCGATTATCCTT