Research Article
TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p
Figure 1
The characterization of MenSCs and MenSC-derived small EVs. (a) The expression of MenSC surface molecules was detected by flow cytometry. Among them, IgG1 antibody is the isotype control of CD29, CD34, CD45, CD73, CD90, CD105, and CD117, and IgG2a is the isotype control of HLA-DR. (b) Optical images of the trilineage differentiation staining and morphology of MenSCs. (c) Transmission electron micrographs of small EVs. (d) Nanoparticle tracking analysis (NTA) of MenSCs-sEV and MenSCs-sEVTNF-α. (e) Western blotting was used to measure the expression of CD63, TSG101, and CD81 molecules on the surface of MenSCs and their small EVs.
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