Research Article
TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p
Figure 2
TNF-α-pretreated MenSC-derived small EVs can relieve colonic inflammation induced by DSS. Male wild-type C57BL/6 mice aged 6-8 weeks were given 5% DSS for one week and intraperitoneally injected with MenSCs-sEV and MenSCs-sEVTNF-α (200 g/mouse) () on day 2. (a, b) DAI scores and the weight of the mice were recorded daily. (c) Neutrophil infiltration in the colon can be measured with the MPO activity detection kit. (d, e) On day 8, after the mice were euthanized, colon length data and gross colon images were obtained. (f) After soaking in 4% paraformaldehyde for 48 hours, the colon was dehydrated and stained with hematoxylin and eosin. Original magnification: ×100 (up) and ×200 (down).
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