Research Article
TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p
Figure 6
MenSCs-sEVTNF-α can increase the proportion of M2 macrophages in vivo. (a) The level of Arg1 and iNOS in the colon of mice was measured by ELISA. (b) Flow cytometry showed the proportion of PKH26+ cells (absorbing PKH26-labeled MenSCs-sEVTNF-α) and M2 macrophages in colonic lamina propria mononuclear cells. (c) PKH26-labeled small EVs were intraperitoneally injected into mice, and the immunofluorescence staining of DAPI, F4/80, and Arg1 in the colon of mice was shown in the confocal microscope.
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