TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p

Figure 7

Expression difference analysis of microRNA in MenSCs-sEV and MenSCs-sEV^TNF-α. (a) Heat map of microRNA expression difference between MenSCs-sEV and MenSCs-sEV^TNF-α of microRNA sequence. (b) KEGG was used to analyze the signaling pathways involved in the differential expression of microRNA between MenSCs-sEV and MenSCs-sEV^TNF-α. (c) MicroRNA sequencing results were verified by qPCR. After 0, 1, 2, 3, and 4 days of TNF-α pretreatment, the expression value of miR-24-3p relative to u6 increased with the pretreatment days. (d, e) The difference of microRNA expression between MenSCs-sEV^TNF-α and MenSCs-sEV was presented by the volcano map and microRNA scatter map. (f) The viability of MenSCs treated with TNF-α of different concentrations was detected by the CCK8 kit.

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