TNF-α Enhances the Therapeutic Effects of MenSC-Derived Small Extracellular Vesicles on Inflammatory Bowel Disease through Macrophage Polarization by miR-24-3p

Figure 8

MenSCs-sEV^TNF-α promotes the polarization of M2 macrophages through miR-24-3p and its downstream IRF1. (a) Luciferase reporter gene assay showed that miR-24-3p could bind to site 1 of IRF1. (b) Representative flow cytometry images showed the proportion of M1(F4/80+iNOS+) and M2(F4/80+CD206+) macrophages in RAW264.7 cells with different treatments. (c) The protein expression of IRF1 was presented by Western blotting after intraperitoneal injection of MenSCs-sEV^TNF-α in DSS-induced acute IBD mice. (d) Western blotting images of RAW264.7 with three treatments, including control, LPS+PBS, and LPS+MenSCs-sEV^TNF-α. (e) The effects of si-IRF1 and IRF1 OE plasmid transfection on the expression of IRF1 in RAW264.7 were verified by Western blotting. (f) After processing RAW264.7 with LPS and knockdown or overexpression (OE) of miR-24-3p and IRF1, the Western blotting image of iNOS and Arg1 was obtained.

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