Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells

Figure 2

µMOs show similar cell type composition to in vivo midbrain. (a–c) Confocal images of µMOs. Scale bar, 20 μm. (d and e) Confocal images of µMOs showing glutamatergic neurons and GABAergic. Scale bar, 20 μm. (f–h) Confocal images of µMOs showing astrocyte marker S100β (f), GFAP (g), and AQP4 (h). Scale bar, 100 μm. (i) Confocal images showing the expression pattern of oligodendrocyte markers in µMOs. Scale bar, 20 μm. (j) Confocal images of µMOs showing mature mDA neurons. Scale bar, 20 μm.

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