Production of Highly Uniform Midbrain Organoids from Human Pluripotent Stem Cells

Figure 4

Minimal variability in µMOs within and across batches and obtained from different cell lines. (a) Images of individual µMOs produced in 96- (left) and 384-well plates (right) using GFP-labelled hESCs. (b) Representative morphological images of typical MOs (i) and µMOs derived from 96- (ii) to 384-well (iii) plates. Scale bar, 1 mm. (c) Average diameter of typical MOs and µMOs from H1 hESCs, H9 hESCs, and hiPSCs (DIV 30), 20 typical MOs or µMOs from each cell line were used for analysis. Data are presented as mean ± SD from three independent experiments. (d) Overview of high-content imaging showing the expression patterns of TH and MAP2 in 96 individual µMOs from 96-well plate. Scale bar, 100 μm. (e) Representative examples of high-content imaging. Scale bar, 100 μm. (f and g) Brightness of TH (f) and MAP2 (g) signals in 96 individual µMOs. The continuous line represents the mean value of 96 individual µMOs from a 96-well plate and the dotted lines show the range of SD.

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