Establishment of Epithelial Inflammatory Injury Model Using Intestinal Organoid Cultures

Figure 4

TJP expressions of intestinal organoid with TNF-α treatment. qRT-PCR analyses of Ocln (a) and Cldn1 (b) mRNA in organoids cultured under the indicated conditions ( well). values were determined by two-sided unpaired Student’s -test. Gapdh was used as a control to normalize the expression of target genes. ; ; . (c) Immunofluorescence staining of representative TJPs of intestinal organoids, including Ocln (first row, green) and Cldn1 (second row, green). Scale  μm. The experiments were independently repeated at least three times with similar results. Ocln: occludin; Cldn1: claudin-1.

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