Mesenchymal Stem Cells Inhibit Epithelial-to-Mesenchymal Transition by Modulating the IRE1α Branch of the Endoplasmic Reticulum Stress Response

Figure 1

Transforming growth factor-β1 (TGF-β1) induced Epithelial–mesenchymal transition (EMT) in A549 cells. (a) Effects of TGF-β1 (5, 10, 20 ng/ml) on the viability of A549 cells after 24, 48 and 72 hr (n = 3, one-way ANOVA with Dunnett’s test). (b) Phase-contrast images of A549 cells in the different groups. Scale bar, 100 μm. (n = 3). (c) The protein expression levels of E-cadherin and vimentin were measured using western blotting, and the results were quantified using densitometry in ImageJ software (n = 3, one-way ANOVA with Duncan’s post hoc test). (d) Images of immunofluorescence staining of E-cadherin (green) and vimentin (red) in A549 cells in the different groups. Scale bar, 50 μm. The data are shown as the means ± SEM (, vs. the control group).

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