Inhibition of Autophagy Maintains ESC Pluripotency and Inhibits Primordial Germ Cell Formation in Chickens

Figure 5

Baf-A1 inhibited the formation of PGCs in vivo. (a, b) After rapamycin (autophagy activator) or Baf-A1 (autophagy inhibitor) was injected in vivo, the expression of p62 and LC3B was detected using qRT-PCR and western blot. (c, d) The expression of reproductive marker genes was detected using qRT-PCR. (e) After labelling positive cells with CVH (PGCs specific protein), the efficiency of PGCs formation after autophagy activation or inhibition in vivo was detected by flow cytometry. (f) After rapamycin (autophagy activator) or Baf-A1 (autophagy inhibitor) was injected in vivo, the number of PGCs in chicken embryos between groups was calculated using glycogen staining of paraffin sections. Scale  μm.

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