Inhibition of Autophagy Maintains ESC Pluripotency and Inhibits Primordial Germ Cell Formation in Chickens

Figure 6

Baf-A1 inhibited the formation of PGCs in vitro. (a, b) After rapamycin (autophagy activator) or Baf-A1 (autophagy inhibitor) was added in vitro, the expression of p62 and LC3B were detected using qRT-PCR and western blot. (c, d) After the autophagy activity was altered in vitro, the expression of reproductive marker genes were detected using qRT-PCR , . (e, f) Morphological observation of the embryoid body in vitro after activation or inhibition of autophagy. Scale  μm. (g) iPGCs were stained with a rabbit monoclonal antibody against CVH. (h) Flow cytometry assays the efficiency of PGCs formation after autophagy was activated or inhibited in vitro.

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