Extracellular Vesicles Derived from Three-Dimensional-Cultured Human Umbilical Cord Blood Mesenchymal Stem Cells Prevent Inflammation and Dedifferentiation in Pancreatic Islets

Figure 6

Effect of 3D hUCB-MSC-derived EVs on M1/M2 polarization of islet-resident macrophages in islets. The hIAPP^+/- mouse islets were cultured under four conditions: medium supplemented with 10% fetal bovine serum (FBS group), medium supplemented with 0.625% bovine serum albumin (BSA group), 20 μg/mL 2D hUCB-MSC-derived EVs plus 0.625% BSA (BSA+2D EV group), and 20 μg/mL 3D hUCB-MSC-derived EVs plus 0.625% BSA (BSA+3D EV group). (a) Immunocytochemical staining with antibodies against insulin (red), DAPI (blue), and ARG1, CD80 and IL-1β (green). Scale . (b) The percentage proportions of ARG1-, CD80-, and IL-1β-stained cells (green) among total DAPI-positive islet cells (blue; 50~100 islets per group). The error bars represent the standard deviation of measurements in three separate sample runs (). Columns, mean; bars, SD. , , , and by one-way ANOVA and Tukey’s posttest.

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