Extracellular Vesicles Derived from Three-Dimensional-Cultured Human Umbilical Cord Blood Mesenchymal Stem Cells Prevent Inflammation and Dedifferentiation in Pancreatic Islets

Figure 7

Effect of 3D hUCB-MSC-derived EVs on the expression of β-cell identity markers in islets. The hIAPP^+/- mouse islets were cultured under four conditions: medium supplemented with 10% fetal bovine serum (FBS group), medium supplemented with 0.625% bovine serum albumin (BSA group), 20 μg/mL 2D hUCB-MSC-derived EVs plus 0.625% BSA (BSA +2D EV group), and 20 μg/mL 3D hUCB-MSC-derived EVs plus 0.625% BSA (BSA +3D EV group). (a) Concentrations of Oct4, NGN3, Pdx1, and FoxO1 in hIAPP^+/- islet cells treated with EVs produced by 2D- or 3D-cultured hUCB-MSCs were measured using qRT-PCR. The error bars represent the standard deviation of measurements in three separate sample runs (). (b) Immunocytochemical staining with antibodies against insulin (red); DAPI (blue); and Oct4, NGN3, Pdx1, and FoxO1 (green). Scale . (c) The percentage proportions of Oct4-, NGN3-, Pdx1-, and FoxO1-stained cells (green) among total DAPI-positive islet cells (blue; 50~100 islets per group). The error bars represent the standard deviation of measurements in three separate runs (). (d) Immunocytochemical staining with antibodies against insulin (red), DAPI (blue), and hIAPP oligomer and cleaved caspase-3 (green). Scale . (e) The percentage proportions of hIAPP oligomer and cleaved caspase-3-stained cells (green) among total DAPI-positive islet cells (blue; 50~100 islets per group). The error bars represent the standard deviation of measurements in three separate sample runs (). Columns, mean; bars, SD. , , , and , by one-way ANOVA and Tukey’s posttest.

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