Adipose-Derived Stem Cell Exosomes Antagonize the Inhibitory Effect of Dihydrotestosterone on Hair Follicle Growth by Activating Wnt/β-Catenin Pathway

Figure 6

ADSC-Exos activates the Wnt/β-catenin pathway by promoting pGSK-3β (Ser9) and β-catenin expression and β-catenin nuclear translocation. (a) ADSC-Exos upregulates the expression of pGSK-3β and β-catenin. (b) Relative quantification of protein bands in grayscale values. (c) Immunofluorescence staining of β-catenin after treatment of DPCs with 0, 5, 10, 20 μg/mL of ADSC-Exos, scale bar: 50 μm. (d) Expression levels of GSK-3β, pGSK-3β, and β-catenin and nuclear transfer of β-catenin in hair follicles of each group by immunofluorescence, scale bar: 100 μm. (e–g) Mean fluorescence intensity of total β-catenin, nuclear β-catenin, and pGSK-3β/ GSK-3β in hair matrix region. (ns: , , , and ).

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