The m<sup>6</sup>A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway

<div>Knockdown of YTHDF1 compromised osteogenic differentiation of hBMSCs <i>in vitro</i>. (a) The expression of osteogenic markers (RUNX2, ALP, COL1A1, and osterix) was evaluted by qRT–PCR 3 days after osteogenic culture of hBMSCs knocked down by siYTHDF1. (b) Western blot analysis of osteogenic markers (RUNX2, COL1a1, and SP7) on Days 1 and 3 of osteogenic differentiation of hBMSCs. (c) Alkaline phosphatase activity was evaluated by ALP staining at Day 5 of osteogenic differentiation. Scale bar: 200 <i>μ</i>m. (d) Mineral deposits were determined by ARS staining on Day 14 of osteogenic differentiation. Scale bar: 200 <i>μ</i>m. (e) Immunofluorescence staining for the COL1A1 and RUNX2 protein after 3 days of osteogenesis. 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Stem Cells International

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Figure 3

Figure 3: The m<sup>6</sup>A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway 

Figure 3 | The m6A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway 