The m<sup>6</sup>A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway

<div>Upregulation of YTHDF1 promoted osteogenic differentiation of hBMSCs <i>in vitro</i>. (a) The expression of osteogenic markers (RUNX22, ALP, COL1A1 and osterix) was evaluated by qRT–PCR 3 days after osteogenic differentiation. (b) Western blot analysis of osteogenic markers after osteogenic differentiation for 1 and 3 days. (c) Alkaline phosphatase activity was evaluated by ALP staining at Day 5 of osteogenic differentiation. Scale bar: 200 <i>μ</i>m. (d) Mineral deposits were determined by ARS staining on Day 14 of osteogenic differentiation. Scale bar: 200 <i>μ</i>m. (e) Immunofluorescence staining for the COL1A1 and RUNX2 protein after 3 days of osteogenesis. 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Stem Cells International

fig5

Figure 5

Figure 5: The m<sup>6</sup>A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway 

Figure 5 | The m6A Reader YTHDF1 Accelerates the Osteogenesis of Bone Marrow Mesenchymal Stem Cells Partly via Activation of the Autophagy Signaling Pathway 