Human Umbilical Cord Mesenchymal Stem Cells Inhibit Pyroptosis of Renal Tubular Epithelial Cells through miR-342-3p/Caspase1 Signaling Pathway in Diabetic Nephropathy

Figure 6

UC-MSC-derived miR-342-3p inhibits NLRP3 inflammasome activation in vitro through targeting the 3-UTR of Caspase1. (a) Putative conserved binding sites of Caspase1 with miR-342-3p and the seed sequence of miR-342-3p. (b) Luciferase activity in HEK293T transfected with constructed plasmids. (c) The expression of miR-342-3p in control, NC mimic-transfected, and miR-342-3p mimic-transfected UC-MSCs. The supernatant was collected as UC-MSC-CM. (d–i) Protein expression of NLRP3, Caspase1, and GSDMD was assayed by western blot. The data were representative of the results of three independent experiments. Data are expressed as . ANOVA was used for comparison among multiple groups. and .

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