lncRNA CYTOR Facilitates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating SOX11 via Sponging miR-6512-3p

Figure 2

CYTOR directly binds to miR-6512-3p. (a) The Venn diagram showed the overlap of miRNAs which had potential binding sequences of CYTOR, based on several online systems. (b) The miR-6512-3p levels throughout the osteogenic differentiation time course were detected by qRT-PCR. (c) The putative binding sites (blue color) and mutated binding sites (red color) between CYTOR and miR-6512-3p were shown. The dual luciferase reporter constructs containing the wild type (CYTOR-wt) or mutant CYTOR (CYTOR mut). (d) CYTOR-wt or CYTOR mut was cotransfected into 293T cells with miR-6512-3p mimic or negative control, and Luciferase activity was detected. (e) RNA-binding protein immunoprecipitation (RIP) assay was performed by using the AGO2 antibody in PDLSCs. The enrichment of CYTOR and miR-6512-3p in AGO2-immunoprecipitated complexes were assessed by following qRT-PCR. (f) Positive control of RIP procedure. Anti-SNRNP70 served as a positive control antibody. (g) Level of miR-6512-3p was assessed by qRT-PCR in overCYTOR or overCYTOR mut MRE-treated hPDLSCs. , , and , compared with the control group as indicated.

(a)

(b)

(c)

(d)

(e)

(f)

(g)