Galectin-3 Plays an Important Role in BMP7-Induced Cementoblastic Differentiation of Human Periodontal Ligament Cells by Interacting with Extracellular Components

Figure 6

Depletion of galectin-3/CM3 in hPDLSCs inhibits BMP7-induced cementoblastic differentiation. Endogenous galectin-3 was depleted using siRNA construct as indicated in Materials and Methods. The protein levels of endogenous galectin-3 and the cell morphologies are shown in (a, b). 1, undifferentiated hPDLCs; 2, hPDLCs treated with BMP7 and SB431542 for 9 days; 3, hPDLC treated simultaneously with BMP7, SB431542, and siRNA constructs. (c) Transcriptional expression of the representative cementoblastic markers. Statistical significance is determined using Student’s -test (). ; ; .

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