Galectin-3 Plays an Important Role in BMP7-Induced Cementoblastic Differentiation of Human Periodontal Ligament Cells by Interacting with Extracellular Components

Figure 8

Galectin-3 interacts with laminin α2 or BMP7 in cementoblast-like cells. (a) Endogenous protein level of laminins in TGF-β1-induced PDL fibroblasts (lane 1) and BMP7-induced cementoblast-like cells (lane 2). LAMA2: laminin α2; LAMB3: laminin β3; LAMC3: laminin γ3. (b) GB1107 effect on the interaction between galectin-3 and laminin α2. Laminin α2 was detected in the immunoprecipitates of galectin-3 (α-Gal3 IP/α-LAMA2 IB). Endogenous protein amounts of galectin-3 and laminin α2 in total cell extracts (CE) were not changed due to GB1107 treatment. Lane 1, no treatment with GB1107 (-GB); lane 2, treatment with GB1107 (+GB). (c) GB1107 effect on the interaction between galectin-3 and ectopic BMP7. BMP7 expressed ectopically was detected in the immunoprecipitates of galectin-3 (α-Gal3 IP/α-FLAG IB). Endogenous protein amounts of galectin-3 and ectopic BMP7 in HeLa cell extracts (CE) were not changed due to GB1107 treatment. Lane 1, no expression of BMP7; lane 2, cells with BMP7 cDNA expression without GB1107 treatment; lane 3, cells with BMP7 cDNA expression with GB1107 treatment.

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