[Retracted] Nucleus Pulposus Cells Induce M2 Polarization of RAW264.7 via CX3CL1/CX3CR1 Pathway and M2 Macrophages Promote Proliferation and Anabolism of Nucleus Pulposus Cells

Figure 9

Coculture system of M2 macrophages and NPC was established. M2 macrophages reduced the apoptosis rate of NPC although did not reach a significant level as measured by TUNEL staining (a). However, the reduction of apoptosis rate reached the significant level in AO/EB staining assay (b) and flow cytometry (d). CCK-8 assay revealed that M2 macrophages significantly increased the NPC viability (c). Meanwhile, the expression level of PCNA gene increased, and the ratio of Bac/Bcl-2 genes decreased in NPC (e). Moreover, the expression level of COL-2 and ACAN significantly increased in NPC, while the expression level of TNF-α, IL-6, and MMP-3 significantly decreased in NPC (G). “” indicates , “” indicates . “” indicates . RAW: RAW264.7 macrophages. NPC: nucleus pulposus cells.

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