BMSC-Derived Exosomes Alleviate Sepsis-Associated Acute Respiratory Distress Syndrome by Activating the Nrf2 Pathway to Reverse Mitochondrial Dysfunction

Figure 2

Sepsis-subjected AECII apoptosis was closely positive with mitochondrial dysfunction. (a) The mice were subjected to CLP surgery for 24 and 48 hours. Primary AECIIs were isolated with flow cytometry and stained with Annexin V and 7-AAD. The percentage of double Annexin V- and 7-AAD-positive cells after 48 hours of CLP was almost 16%. (b) The AECIIs (MLE-12 cells) were exposed to LPS (25 μg/ml) for 6, 24, and 48 hours. The percentage of double Annexin V- and 7-AAD-positive cells was detected by flow cytometry. (c) The expression of mitochondrial biogenesis-related proteins (PGC-1α, Tfam, and Nrf1) was detected with western blotting. (d) The mitochondrial ATP content was detected by an ATP fluorometric assay kit. (e, f) Mitochondrial fission protein p-Drp1 and antioxidant Nrf2 protein expressions were detected using western blotting. Data are represented as , per group. NS: nonsignificance; or vs. the control group.

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