Research Article
BMSC-Derived Exosomes Alleviate Sepsis-Associated Acute Respiratory Distress Syndrome by Activating the Nrf2 Pathway to Reverse Mitochondrial Dysfunction
Figure 3
BMSC-exos reversed mitochondrial dysfunction by activating the Nrf2 pathway. AECIIs were pretreated with Nrf2 inhibitor-ML385 (20 μM) for 30 min, followed by LPS (25 μg/ml) for 1 hour, and subsequently cocultured with BMSC-exos (100 μg/ml) for 48 hours. (a, b) Expressions of Nrf2, PGC-1α, Nrf1, and Tfam proteins were detected with western blotting. (c) Mitochondrial ATP content in AECIIs was measured with an ATP fluorometric assay kit. (d) Expression of the p-Drp1 protein was detected by western blotting. (e) MitoTracker Green-stained mitochondrial morphology in AECIIs was observed with immunofluorescence. (f) The average length of the mitochondria in each group was analyzed. Data are represented as , per group. NS: nonsignificance; vs. the control group; ### vs. the LPS group.
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