BMSC-Derived Exosomes Alleviate Sepsis-Associated Acute Respiratory Distress Syndrome by Activating the Nrf2 Pathway to Reverse Mitochondrial Dysfunction

Figure 4

BMSC-exos alleviated sepsis-treated AECII apoptosis via activating the Nrf2 pathway in vivo and in vitro. AECIIs were pretreated with ML385 (20 μM) for 30 min, followed by LPS (25 μg/ml) for 1 hour, and subsequently cocultured with BMSC-exos (100 μg/ml) for 48 hours. (a) The frequency of double Annexin V- and 7-AAD-positive AECIIs was characterized by flow cytometry. The mice were intraperitoneally injected with ML385 (30 mg/kg) 2 hours before CLP surgery and BMSC-exosomes (300 μg/mouse) 1 hour before CLP surgery. (b) TUNEL staining (brown) was detected in the lung sections of all the treated mice. Data are represented as the , per group. NS: nonsignificance; vs. the control group; ^### vs. the LPS group or CLP group.

(a)

(b)