Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation

Figure 2

Brn4 binds to the promoters of Lama2 and Samsn1 and promotes their expression. (a) Overlapping genes of RNA-seq and ChIP-seq. (b, c) Representative immunoblots of Brn4-HA and β-actin in the N2a stable Brn4-overexpressed lentivirus strains (b) and quantification of Brn4-HA protein level normalized to β-actin (c). (d) Quantification of Brn4 mRNA level normalized to β-actin. (e) Quantification of mRNA levels for the overlapping genes between RNA-seq and ChIP-seq normalized to β-actin. (f) Nucleic acid electrophoresis for ChIP-PCR assay in the N2a stable Brn4-overexpressed lentivirus strains. (g) Sequences of the top three Brn4 binding sites on the Lama2 and Samsn1 promoters with the highest scores. (h, i) Dual-luciferase reporter gene experiment using N2a cells. (j–m) Representative immunoblots of Lama2, Samsn1, and β-actin in the N2a stable Brn4-overexpressed lentivirus strains (j, k) and quantification of Lama2 and Samsn1 protein levels normalized to β-actin (l, m). Error bars represent the . . , . Abbreviations: Ctrl: control; Brn4-OE: Brn4-overexpression.

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