Research Article
Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung
Table 2
Surface markers used to identify immune cells by flow cytometry and immunofluorescence.
| Cell type | Flow cytometry | Immunofluorescence | Reference |
| Granulocytes | Cd11c− CD24hi | Myeloperoxidase (MPO) | [41] | Neutrophils | Siglec F− CD11bhi CD103− Ly6Ghi | MPO + Ly6G | [41] | Monocyte/M0 macrophages | CD11bhi MHC II+/− CD64+/− | N/A | [41] | Alveolar macrophages | Cd11b− CD11chi CD64+ | N/A | [33] | Interstitial macrophages | MHC II+ CD11b+ CD64+ CD24− | N/A | [42] | Proinflammatory monocytes | CD11bhi MHC II+/− CD64hi CD71− | Cd11b + Ly6C | [43] | Anti-inflammatory monocytes | CD11bhi MHC II+/− CD71hi | N/A | [44] | Proinflammatory macrophages | CD11chi CD11b+/− CD24− CD64hi CD71− | F4/80 + Cd16/32 | [45, 46] | Anti-inflammatory macrophages | CD11chi CD11b+/− CD24− CD64− CD71hi | F4/80 + CD206 | [47] |
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