Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung

Table 3

Antibodies used for immunofluorescence.

	Host	Clone	Isotype	Dilution	T°/time	Manufacturer
F4/80 FITC	Human	REA126	IgG1	1 : 50	RT/2 hr	Miltenyi biotech (130-117-509)
CD16/32	Human	REA377	IgG1	1 : 10	RT/2 hr	Miltenyi biotech (130-107-066)
Ly6C	Rat	HK1.4	IgG2c, k	1 : 200	4/ON	Biolegend (128001)
MPO	Goat	Polyclonal	IgG	1 : 100	4/ON	R&D Systems (AF3667-SP)
HDAC2	Rabbit	Polyclonal	IgG	1 : 250	RT/2 hr	Sigma–Aldrich (HPA011727)
CD11b APC	Rat	M1/70.15.11.5	IgG2b, k	1 : 50	RT/2 hr	Miltenyi biotech (130-113-231)
Ly6G APC	Human	REA526	IgG1	1 : 50	RT/2 hr	Miltenyi biotech (130-120-803)
CD163	Rabbit	Polyclonal	IgG	1 : 100	RT/2 hr	Invitrogen (PA5-78961)
Alexa Fluor® 750 donkey antirat	Donkey	Polyclonal	IgG	1 : 200	RT/1 hr	Abcam (ab175750)
Alexa Fluor® 647 goat antirabbit	Goat	Polyclonal	IgG	1 : 1,000	RT/1 hr	Invitrogen (A-212465)
Alexa Fluor® 647 goat antihamster	Goat	Polyclonal	IgG	1 : 1,000	RT/1 hr	Invitrogen (A-21451)
Alexa Fluor® 633 goat antirat	Goat	Polyclonal	IgG	1 : 1,000	RT/1 hr	Invitrogen (A-21094)