Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia

Figure 3

Extracellular vehicles (EVs) derived from human umbilical cord mesenchymal stem cells (hUC-MSCs) elevated HTR8-S/Vneo cell proliferation and migration abilities. (a) HTR8-S/Vneo cell proliferation rate was examined by MTT assay. mRNA levels of PCNA (b) and Ki67 (c) were examined by qRT-PCR. (d) Western blotting was used to measure the protein expressions of PCNA and Ki67. Relative expression was normalized to Blank (e and f). (g) HTR8-S/Vneo cell migration ability by transwell assay (n = 3). and compared to Blank and Mann–Whitney test. Data were presented as mean ± SD. Ki67, nucleus related antigen 67; mRNA, messenger ribonucleic acid; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PCNA, proliferating cell nuclear antigen; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

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