Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia

Figure 5

miR-195 targeted tissue factor pathway inhibitor 2 (TFPI2) and regulated the expression of TFPI2 in HTR8-S/Vneo cells. (a) The suspected binding of mutated 3′-UTR region of TFPI2 mRNA and hsa-miR-195 with the wild type is shown. (b) HEK-293T and HTR8-S/Vneo cells were transfected with indicated luciferase reporters. Relative luciferase activities were measured after incubation for 48 hr, (b and c). (d) HTR8-S/Vneo cells were transfected with negative control or miR-195 mimics for 48 hr. qRT-PCR was used to analyze the expressions of miR-195. (e) HTR8-S/Vneo cells were transfected with miR-195 mimics or negative control for 48 hr. Western blotting was used to measure the protein expressions of TFPI2. n = 3. Data were presented as mean ± SD. and . One-way analysis of variance (ANOVA) followed Tukey’s multiple comparisons test. mRNA, messenger ribonucleic acid; miR-195, microRNA-195; qRT-PCR, quantitative reverse transcription polymerase chain reaction; UTR, untranslated region.

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