Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia

Figure 6

Elevated extracellular vesicle (EV)-derived miR-195 from human umbilical cord mesenchymal stem cell (hUC-MSCs) boosted HTR8-S/Vneo cell proliferation and migration. (a) Detection of HTR8-S/Vneo cell proliferation in each group by MTT assay. qRT-PCR was used to analyze the mRNA expressions of Ki67 (b) and PCNA (c). Relative expressions were normalized to blank-EVs. (d) Detection of HTR8-S/Vneo cell migration ability by transwell assay and the ratio of cell migration. n = 3. Data were presented as mean ± SD. and compared to blank-EVs. One-way analysis of variance (ANOVA) followed Tukey’s multiple comparisons test. Ki67, nucleus related antigen 67; mRNA, messenger ribonucleic acid; miR-195, microRNA-195; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PCNA, proliferating cell nuclear antigen; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

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