The Effect of Mesenchymal Stem Cell-Derived Exosomes and miR17-5p Inhibitor on Multicellular Liver Fibrosis Microtissues

Figure 3

Characterization of umbilical cord mesenchymal stem cell-derived exosomes (UC-MSC-EXO). (a) UC-MSC at Passage 3. (b) Osteogenic differentiation of UC-MSC stained by alizarin red S. (c) Adipogenic differentiation of UC-MSC stained by Oil red O (scale bars: a 200 μm, b and c 100 μm). (d) TEM photograph of UC-MSC-derived exosome (scale bar: 300 nm). (e) Dynamic light scattering analysis certified the size of exosomes. (f) Identification of specific surface markers of UC-MSC-exosome including CD9 and CD81 by flow cytometry. (g) PKH-labeled vesicle internalization in multicellular liver microtissues (MLMs) (scale bar: 100 μm). Abbreviation: UC-MSC-EXO, umbilical cord mesenchymal stem cell-derived exosomes; MLMs, multicellular liver microtissues.

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