Research Article

Human Infrapatellar Fat Pad Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibit Fibroblast Proliferation by Regulating MT2A to Reduce Knee Arthrofibrosis

Figure 1

Identification of infrapatellar fat pad mesenchymal stem cells (IPFSCs) and IPFSC-EVs. (a) IPFSCs were isolated and cultured, showing a spindle-like shape under optical microscopy. (b) IPFSCs had adipogenic, osteogenic, and chondrogenic differentiation ability. (c) Characteristic antigens of IPFSCs detected using flow cytometry. (d) IPFSC-EVs showed a typical disc-shaped bilayer membrane structure under transmission electron microscopy. (e) NanoSight analysis showing the diameter range of the extracted EVs. (f) Western blot showing the expression levels of the characteristic EV proteins CD63 and CD81. (g) After PKH26 labeling of IPFSC-EVs, uptake of IPFSC-EVs by fibroblasts was observed under fluorescence microscopy.
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