Human Infrapatellar Fat Pad Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibit Fibroblast Proliferation by Regulating MT2A to Reduce Knee Arthrofibrosis

Figure 2

IPFSCs-EV treatment reduced the severity of knee arthrofibrosis in rats. (a) Hematoxylin and eosin staining showed that IPFSC-EVs reduced the degree of fibrosis and fibroblast number in the knee joint cavity. Masson staining showed that IPFSC-EVs reduced collagen content in the fibrotic tissues. (c and d) Immunohistochemical staining of collagens I and III revealed that IPFSC-EVs reduced collagen content in the fibrotic tissues in a concentration-dependent manner. (e and f) Immunohistochemical staining of IL-6 and TNF-α showed that IPFSC-EVs reduced the expression of proinflammatory factors in the fibrotic tissues in a concentration-dependent manner. (g) Immunohistochemical staining of α-SMA also revealed that IPFSC-EVs reduced the degree of fibrosis in the knee joint cavity in a concentration-dependent manner.

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