Human Infrapatellar Fat Pad Mesenchymal Stem Cell-Derived Extracellular Vesicles Inhibit Fibroblast Proliferation by Regulating MT2A to Reduce Knee Arthrofibrosis

Figure 4

IPFSC-EVs inhibited fibroblast proliferation in the inflammatory environment. (a) Western blot and RT–PCR showing that IPFSC-EVs could promote MT2A expression. (b) Cell cycle analysis showing that IPFSC-EVs could arrest fibroblasts in the G1 phase in the inflammatory environment and inhibit cell cycle progression. (c and d) Western blot and RT–PCR results showing downregulated mRNA and protein expression levels of the proliferation-related genes CCND1 and PCNA after IPFSC-EV treatment of fibroblasts. (e) EdU cell proliferation assay showing a significantly lower proportion of EdU-positive cells in total cells after IPFSC-EV treatment of fibroblasts in the inflammatory environment for 24 h. All data are presented as . compared with the control group.

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