Abnormal Decrease of Macrophage ALKBH5 Expression Causes Abnormal Polarization and Inhibits Osteoblast Differentiation

Figure 1

Macrophage M1/M2 polarization imbalance in type II diabetes. (a) The percentages of M1 type macrophage (CD68-CCR2) in type II diabetic mice were analyzed by flow cytometry. (b) Quantification of percentages of M1 type macrophage (CD68-CCR2) in type II diabetic mice. (c) The percentages of M1 type macrophage (CD68-CCR7) in type II diabetic mice were analyzed by flow cytometry. (d) Quantification of percentages of M1 type macrophage (CD68-CCR7) in type II diabetic mice. (e) The percentages of M2 type macrophage (CD163-CX3CR1) in type II diabetic mice were analyzed by flow cytometry. (f) Quantification of percentages of M2 type macrophage (CD163- CX3CR1) in type II diabetic mice. (g) The percentages of M2 type macrophage (CD163-CD206) in type II diabetic mice were analyzed by flow cytometry. (h) Quantification of percentages of M2 type macrophage (CD163-CD206) in type II diabetic mice. (i) qPCR analysis of IL-1b, IL-6, and Tnf mRNA levels in type II diabetic mice. (j) qPCR analysis of Arg1, IL-10, and Mcr1 mRNA levels in type II diabetic mice. Significance was defined as .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)