Abnormal Decrease of Macrophage ALKBH5 Expression Causes Abnormal Polarization and Inhibits Osteoblast Differentiation

Figure 2

Peri-implantitis combined with type II diabetes is associated with a large imbalance in macrophage M1/M2 polarization. (a) H&E staining of soft tissue from peri-implantitis in type II diabetic and control mice. Scale bar, 50 μm. (b) Representative images of iNOS (green), CD206 (red), and DAPI nuclear staining (blue) soft tissue from peri-implantitis in type II diabetic and control mice. Scale bar, 50 μm. (c and d) Quantification of percentages of iNOS⁺ (c) and CD206⁺ (d) cells in different treatment groups. (e) IL-1b, IL-6, and Tnf expression were examined by qPCR and normalized to GAPDH expression. (f) Arg1, IL-10, and Mcr1 expression were examined by qPCR and normalized to GAPDH expression. Significance was defined as .

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