Umbilical Cord-Derived Mesenchymal Stem Cells Attenuate S100-Induced Autoimmune Hepatitis via Modulating Th1 and Th17 Cell Responses in Mice

Figure 3

Human UC-MSCs prevent CD4⁺ T cells from infiltrating the liver and reduce splenic Th1 and Th17 cell differentiation. (a and b) Mice were sacrificed on day 35 after the first immunization. Immunofluorescence staining showing CD4⁺ T cell infiltration in liver sections from each group. Quantitative analysis of fluorescence imaging using ImageJ software (n = 5) ( , ), (c and d) splenic Th17 cells (CD4⁺ IL-17A⁺) examined using FACS for each group (n = 5) ( , ), and (e and f) splenic Th1 cells (CD4⁺ IFN-γ⁺) examined using FACS for each group (n = 5) ( , ).

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