HMGB1 Modulates High Glucose-Induced Erroneous Differentiation of Tendon Stem/Progenitor Cells through RAGE/β-Catenin Pathway

Figure 1

The osteogenic and tenogenic differentiation capacity of TSPCs under high glucose treatment. (a) Representative images of ARS staining of TSPCs in BM (A–F) and OIM (G–L) under normal glucose (5.5 mM) and high glucose (15 and 25 mM) on day 14. (b) Quantification of ARS bound to the calcium nodules in TSPCs. (c–e) Relative mRNA levels of osteogenic genes in TSPCs cultured in BM or OIM under normal glucose or high glucose for 14 days by qRT-PCR. (f–h) Relative mRNA levels of tendon-related genes in TSPCs by qRT-PCR. A–C, G–I × 1; D–F, J–L × 40. .

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