Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid-Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis

Figure 5

hUC-MSC-CM treatment reduces cell apoptosis and promotes cell proliferation in GES-1: (a, b) the improvement effect of MSC-CM with different concentrations (0.1%, 0.5%, 1%, 5%, 10%, 20%, and 40%) on TNF-α and SM-164-induced cell apoptosis in GES-1 cells; (c, d) TUNEL assay was used to detect the effects of MSC-CM on the apoptosis rate of GES-1 cells which induced by TNF-α and SM-164, and the percentage of TUNEL-positive nuclei was calculated (scale bars = 100 μm); (e) western blot was used to detect the apoptosis-associated protein caspase3 and PARP in GES-1 cells; (f–i) MSC-CM promotes cell proliferation in GES-1 cells; (j, k) MSC-CM promotes cell cycle progression in GES-1 cells.

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