The Neural Progenitor Cell-Associated Transcription Factor FoxG1 Regulates Cardiac Epicardial Cell Proliferation

Figure 4

Snord116 expresssion in rat epicardial cells and human EPDC. (a) TGF-β1 treatment of cultured adult rat epicardial cells decreased Snord116 transcript levels. Lane 1, vehicle-treated cells, Snord116 probe/primers; lane 2, vehicle-treated cells, Snord116 probe/primers, no RT control; lane 3, TGF-β1-treated cells, Snord116 probe/primers; lane 4, vehicle-treated cells, U1 snRNA probe/primers; lane 5, TGF-β1-treated cells, U1 snRNA probe/primers. Note: U1 snRNA serves as a housekeeping gene for normalization of loading. (b) Top: phase contrast photomicrographs of primary human EPDC (passage 1) following EMT in medium containing 10% FCS. Cells were incubated under normoxic or hypoxic (1% oxygen) conditions for 48 hr. bottom: by real-time qRT-PCR assays, Snord116 expression was significantly reduced under hypoxic conditions. n = 3 adult human donors.

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