The Neural Progenitor Cell-Associated Transcription Factor FoxG1 Regulates Cardiac Epicardial Cell Proliferation

Figure 7

FoxG1 knockdown reduced Snord116 expression and epicardial cell proliferation. (a) Left: lentiviral shRNA-mediated FoxG1 knockdown in rat epicardial cells, as shown by western blot. Right: quantification of FoxG1 protein demonstrated 40% knockdown compared with expression levels in control cells transduced with scrambled shRNA (n = 3–4 transduced cell populations, unpaired Student’s t-test). (b) FoxG1 knockdown epicardial cell lines demonstrated a 79% decrease in Snord116 expression compared with scrambled shRNA control cells (n = 3 transduced cell populations, unpaired Student’s t-test). (c) FoxG1 knockdown significantly reduced epicardial cell proliferation. Two-way ANOVA with repeated measures and multiple comparisons. Mean ± SEM plotted in all graphs. and .

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