Exendin-4 Exacerbates Burn-Induced Morbidity in Mice by Activation of the Sympathetic Nervous System

Figure 2

Expression of GLP-1R and its function in T cells. (a) The purified murine CD3⁺ (green) T cells were stained with rabbit anti-mouse GLP-1R antibody (red) and further detected by DyLight™ 549-conjugated goat anti-rabbit or mouse IgG. T cells incubated with control IgG did not show nonspecific binding. Nuclei are labeled with DAPI. (b) Flow cytometry of purified T cells by staining with CD3⁺ antibody. (c) T cell function and differentiation were analyzed by culturing splenocytes with ConA in the presence of PBS (ctrl), low (0.3 nmol/L, Ex-L), moderate (3 nmol/L, Ex-M), and high concentration (30 nmol/L, Ex-H) of Ex-4 with or without Prop (1 nmol/L) or exendin (9-39) (Ex-9) (1 μmol/L) pretreatment. T cell proliferation and secretions of IL-2, IL-4, and IFNγ were presented. Data are mean ± SEM (/group). The differences between the groups were analyzed by one-way ANOVA. vs. PBS and vs. Ex-M, by post hoc LSD analysis in one-way ANOVA or Student’s -test.

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