M2c Macrophages Protect Mice from Adriamycin-Induced Nephropathy by Upregulating CD62L in Tregs

Figure 2

M2c macrophages promote the migration of Tregs from the blood to different sites. (a–e) Flow cytometry of Tregs in the blood, spleen, thymus, kidney, and renal draining lymph nodes in animals treated with Tregs or M2c macrophages+Tregs. (f) Immunofluorescence micrographs of Tregs in the blood, spleen, thymus, kidney, and renal draining lymph nodes in animals treated with Tregs or M2c macrophages+Tregs. FITC: fluorescein; GFP: green fluorescent protein; qRT-PCR: quantitative reverse transcription-polymerase chain reaction; RFP: red fluorescent protein.

(a)

(b)

(c)

(d)

(e)

(f)